Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test

Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW;Combo Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Ehrlichia canis antibodies (EHR), Borrelia burgdorferi antibodies (LSH) and Heartworm (CHW) antigens in dog;s serum, plasma and specimen.

Ehrlichiosis

Ehrlichiosis is a contagious disease that affects dogs and is typically spread by the brown dog tick. In the 1970s, when military canines were discovered to be sick upon their return from Vietnam, it initially came to light as a serious illness. Both German Shepherds and Doberman Pinschers appear to be particularly affected by the illness.A rickettsial organism is the causative agent of this illness. Rickettsiae are a particular class of bacteria, or ;genus.; The most frequent rickettsial species linked to ehrlichiosis in dogs is Ehrlichia canis (E. canis), while additional strains of the pathogen are rarely discovered.It has also been known as tropical canine pancytopenia and tracker dog sickness since it first appeared in military dogs in Vietnam.

Many canines are spotted during the acute phase in places where ehrlichiosis is widespread. Infected dogs may experience a fever, enlarged lymph nodes, respiratory distress, weight loss, bleeding abnormalities (spontaneous hemorrhage or bleeding), and sometimes, neurological changes at this period (they may seem unsteady or develop meningitis). Two to four weeks may pass during this period, during which some canines may recover from the illness or go on to the sub-clinical stage.

Borrelia Burgdorferi

One of the germs that causes Lyme disease in people is the bacterial species Borrelia burgdorferi, which belongs to the genus Borrelia and is a kind of spirochete. It constitutes the species complex of Borrelia burgdorferi sensu lato together with a few related genospecies, some of which also cause Lyme disease. Twenty approved and three suggested genospecies are currently present in the complex. The sole known cause of Lyme disease in North America up until 2016 was B. burgdorferi sensu stricto, which is found in both North America and Eurasia. Species of Borrelia are Gram-negative.

Heartworm

Heartworm, a potentially deadly illness that mostly affects canids like domestic dogs, coyotes, foxes, and wolves but can also infect cats and ferrets, is brought on by the nematode worm Dirofilaria immitis.Clinical symptoms vary in intensity and duration of illness in canid hosts infected with D. immitis. In addition to hemolytic anemia, coughing, trouble breathing, exercise intolerance, ascites, an enlarged liver, shock, and even death, this might produce respiratory and vascular symptoms. A mosquito bite from an infected host to the subsequent host is how heartworm is spread. The nematode worms mature and multiply inside the pulmonary arteries of their host.There are no accurate diagnostics for isolated male worm infections, although testing on serum can identify adult female worm antigen in the blood. Upon necropsy, recovered adult or developing worms can be recognized morphologically or by PCR.

Using a sandwich lateral flow immunochromatographic technique, the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test may detect both diseases. A testing window is provided on the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test card to allow for assay running and result readout observation. Before starting the assay, the testing window has an unseen T (test) zone and a C (control) zone. The Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test strip will laterally flow through the surface when the treated sample is inserted into each sample hole on the device. The liquid will then react with the pre-coated Ehrlichia, Borrelia, and heartworm antibodies, in that order. A recognizable T line will show up if the material contains Ehrlichia, Borrelia, and Heartworm antigens. After a sample is applied, the C line should always show as this denotes a legitimate outcome. This allows the gadget to detect Ehrlichia, Borrelia, and Heartworm antigens in the samples with accuracy.

At room temperature or in the refrigerator (2-30 °C), keep the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid exactly as it came in the sealed pouch. Through the specified expiration date on the sealed packet, the test remains stable. The sealed bag is where the test gadget must stay until it is used.

AVOID FREEZING.

Use only until the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid device's expiry date.

Allow the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test apparatus, sample, buffer, and/or controls to acclimate to room temperature (15-30 °C) before testing.

1. A specimen should be collected and handled as described below. Collect the patient dog;s whole blood, centrifuge it to obtain the serum, or put the whole blood into a tube containing anticoagulants to obtain the plasma. Invert the Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test card after removing it from the foil bag.

2. Add 10μL of the prepared sample to the sample well, lining it up with the window CHW. Add 3 drops (or around 100μL) of assay buffer CHW to the sample well next. Activate the timer.

3. Transfer 20 L of the prepared material into a vial of EHR-LSH assay buffer and well stir. Then, respectfully matching the windows EHR, LSH, place 3 drops (or around 120 mL) of the diluted sample into the sample well "S" of the test card. Launch the timer.

5. In 10 minutes, explain the outcome. Results obtained after 15 minutes are deemed invalid.

• Positive: The Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test displays two lines. The control line zone (C) should always have one line, and the test line region (T) should always contain one apparent colored line.

• Negative: The control region only has one colored line (C).In the test line zone, there are no visible colored lines.
• Invalid:;The control line does not display. The most frequent causes of control line failure are insufficient specimen volume or improper procedural procedures. With a fresh Canine Ehrlichia EHR Borrelia Burgdorferi Heartworm CHW Combo Rapid Test gadget, go over the steps again and do the test again.

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