Saliva Drugs Of Abuse Rapid Test Cup

The Saliva Drugs of Abuse Rapid Test Cup is a rapid visual immunoassay for the qualitative detection of drugs of abuse in human oral fluid specimens. This test detects combinations of the following drugs at the concentrations listed below. Specific combinations will vary according to the test in question: 

The Multi-Saliva DOA Rapid Test Cup For AMP/BZO/COC/MTD/MET/MOR/THC is a rapid, oral fluid screening test that can be performed without the use of an instrument. The test utilizes monoclonal antibodies to selectively detect elevated levels of specific drugs in human oral fluid. The Test Cup is intended for use with human oral fluid specimens only.

The Saliva Drugs of Abuse Rapid Test Cup is an immunoassay based on the principle of competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody. During testing, a portion of the oral fluid specimen migrates upward by capillary action. A drug, if present in the oral fluid specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will show up in the test line region of the specific drug strip. The presence of drug above the cut-off concentration in the oral fluid specimen will saturate all the binding sites of the antibody. Therefore, the colored line will not form in the test line region.

A drug-positive oral fluid specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative oral fluid specimen will generate a line in the test line region because of the absence of drug competition. To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred. 

Bring the DOA test cup, specimens, and/or controls to room temperature (15-30°C) before use. Donors should avoid placing anything (including food, drink, gum and tobacco products) in their mouth for at least 10 minutes prior to specimen collection.

1、The oral fluid specimen should be collected using the collector provided with the kit. No other

collection devices should be used with this assay.

2、Instruct the donor to not place anything in the mouth including food, drink, gum, or tobacco products for at least 10 minutes prior to collection.

3、Bring Saliva Drugs of Abuse Rapid Test Cup, specimens, and/or controls to room temperature (15-30°C) before use.

4、Using the provided collection swab, have donor sweep inside of mouth (cheek, gums, tongue) several times, then the sponge will gradually soften as oral fluid is absorbed, and should be completely soft after 3 minutes. Important: Do not bite, suck, or chew on the sponge.

5、Remove collection swab from mouth and insert it sponge first into the cup, pushing until the locking flange locks in place in the bottom of the cup.

6、Test cup upright on flat surface and keep upright while test is running. Wait for the colored bands to appear in test results area. Read results at 10 minutes. 

POSITIVE: Only one colored band appears, in the control region (C). No colored band appears in the Saliva Drugs of Abuse Rapid Test Cup region (T) for the drug in question. A positive result indicates that the drug concentration exceeds the detectable level.

NEGATIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T) for the drug in question. A negative result indicates thatthe drug concentration is below the detectable level.

INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new cup. If the problem persists, discontinue using the kit immediately and contact your local distributor.

Sensitivity

A phosphate-buffered saline (PBS) pool was spiked with drugs to target concentrations of ± 50% cut-off and 25% cut-off and tested with The Multi-Saliva Drugs of Abuse Rapid Test Cup. The results are summarized below.

Specificity

The following table lists the concentrations of compounds (ng/mL) above which identified positive results at 10 minutes.

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