ABO And RhD Blood Grouping Rapid Test

The ABO and RhD Blood Grouping Rapid Test (Whole Blood) is a solid-phase method test cassette for the regular ABO and RhD blood group detection.

Human blood has a variety of blood types, and the most common blood type system is ABO (blood types are divided into A, B, AB and O types according to the presence or absence of A and B antigens in red blood cells). The second is the Rh blood system which divides blood groups into Rh positive and negative based on the presence or absence of D antigen on red blood cells. When the corresponding antigens and antibodies of ABO and RhD blood group meet, red blood cells will have agglutination reaction, resulting in red blood cell hemolysis, which is life-threatening. Therefore, ABO and RhD blood group identification, or ABO and RhD blood group antigen test, is an important

prerequisite for ensuring safe blood transfusion and avoiding related diseases.

Allow the ABO and RhD Blood Grouping Rapid Test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1. Remove the test cassette from the sealed foil pouch and use it as soon as possible. Best results

will be obtained if the assay is performed within one hour.

2. Place the cassette on a clean and level surface.

3. Hold the dropper vertically and transfer 1 drop of whole blood (approximately 10 L) to each

"S" well of test cassette. See illustration below.

4. Add 7-8 drops (approximately 490-560 L) of washing buffer to "Buffer" well in 1 minute after

the specimen is added.

5. Interpret test results in 1 minute on "S" well. Do not interpret the result after 30 minutes.

Note: If a small amount of red blood cells is observed in the "S" well at reading time, please add 2-3

drops of washing buffer to "Buffer" well and read the results immediately.

Note: It is suggested not to use the buffer beyond 6 months after opening the vial.