The detection principle of the hemoglobin meter

Add a hemolytic agent to the diluted specimen, and the red blood cells dissolve to release hemoglobin: HGB+CN-Methemoglobin cyanide (brown red) HiCN is a very stable hemoglobin derivative, and various hemoglobin components in the blood can be transformed and absorbed The peak is 540nm, which enters the hemoglobin colorimetric system, and can report and display the hemoglobin concentration. Cyanide methemoglobin colorimetric method is a standardized hemoglobin determination method recommended by ICSH and WHO. When red blood cells pass through the micropore, a pulse of corresponding size is formed, and the height of the pulse is superimposed, and the specific volume of red blood cells can be obtained after conversion. Some instruments first calculate the average volume of red blood cells based on the height of a single cell, and then multiply it by the number of red blood cells to obtain the specific volume of red blood cells.